Fig. 2.
Developmental toxicity of Plectonema 33−7 and Tolypothrix 30−1−4 extracts. Within 2 dpf, zebrafish embryos exposed at 4- to 32-cell stage to 50 μg/mL of chloroform extracts from Plectonema 33−7 (A, B and D) and 100 μg/mL of chloroform extracts from Tolypothrix 30−1−4 (F), respectively, show inhibition of pharyngulation, including organogenesis (e.g. formation of the eye) and general cell-differentiation, compared to embryos treated with either 50 μL CHCl3/mL of ERS (C and E), as solvent-only control for A, B and D, or 100 μL of CHCl3/mL of ERS (G), as solvent-only control for F. In the case of Plectonema 33−7, there is additionally an apparent lack of migration of melanophores from neural crest (B) relative to the solvent-only control (C and E). Note: D and E are the same magnification.