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. Author manuscript; available in PMC: 2008 Oct 27.
Published in final edited form as: J Comp Neurol. 2005 Dec 19;493(3):370–380. doi: 10.1002/cne.20779

Figure 8.

Figure 8

Protein expression of melatonin receptor subtypes in astrocytes. A. Western blot analysis of cell culture extracts demonstrated the expression of both the Mel1a and Mel1c receptor proteins in chick diencephalic and chick telencephalic astrocytes. B. Protein extracts of mouse diencephalic and telencephalic glia cells expressed both the Mel1a (MT1) and Mel1b (MT2) receptor subtypes. Anti-MelR probes detected multiple proteins, especially in chick extracts. However, a consistent protein band with a molecular weight of 133 kD was present in all samples. Anti-actin immunostaining was used as a protein loading control. These gels represent one of three replicate experiments that produced similar results.