FIGURE 3.

Human MBOAT5 exhibits preference for polyunsaturated acyl-CoAs. Acyltransferase assays were performed as described for Fig. 2. The amount of each individual deuterated PC species is expressed as the percentage of its LC/MS/MS intensity integrated area compared with the endogenous 34:2 PC intensity integrated area. Results shown represent average ± S.E. (vector, n = 3; MBOAT5, n = 6). Inset, acyltransferase activity of MBOAT5-expressing yeast microsomes was measured with radiolabeled 18:1-lysoPC and individual acyl-CoA species containing the indicated fatty acyl chains in separate reactions. The data represent the average ± S.D. of three separate experiments conducted in duplicate. ^*, p < 0.05 (compared with empty vector).