FIGURE 5.

Dual substrate choice assay of lysophospholipid acyltransferases present in ale1Δ yeast microsomes. Microsomes from yeast transformed with empty vector were incubated with a mixture of eight acyl-CoAs (30 μm each) as detailed above and a mixture of six lysophospholipids (lyso-PA, deuterated lyso-PC, lyso-PE, lyso-PG, lyso-PI, and lyso-PS; 10 μm each). The amount of each individual phospholipid species formed in the assay is expressed as the ratio of its integrated LC/MS/MS signal relative to the corresponding phospholipid internal standard (IS) added at the end of the incubation. Data shown are from one experiment representative of two independent experiments.