FIGURE 6.

Dual substrate choice acyltransferase assays demonstrate the preferential utilization of arachidonic acid and thimerosal sensitivity of MBOATs 5 and 7. Acyltransferases were assayed as described above with microsomes from yeast expressing the indicated human proteins either in the absence or in the presence of 50 μm thimerosal. The amount of each individual phospholipid species is expressed as the ratio of its integrated LC/MS/MS signal relative to the corresponding phospholipid internal standard (IS) added at the end of the incubation. Data are normalized for MBOAT expression, and background activity present in yeast transformed with empty vector is subtracted. Data shown are average ± S.E. of two individual experiments performed in triplicate. ^*, p < 0.05 (compared with control).