FIGURE 11.
FLAG-tagged and partnering GABAA receptor subunits were coimmunoprecipitated. Representative immunoblots of proteins immunoprecipitated (IP) from cells with α1β2FLAG, α1(D420A)β2FLAG, and α1β2(D450A)FLAG subunit coexpression using anti-FLAG M2 beads. Immunoprecipitated proteins were detected using monoclonal anti-α1(upper panel) or anti-β2 antibodies (lower panel). Note the 1-kDa shift in molecular mass due to insertion of the FLAG epitope in the β2 subunit (compared with immunoblots of untagged subunits shown in previous figures).