FIGURE 2.

Intracellular localization and insulin responsiveness of IRAP in 3T3-L1 cells. A and C, undifferentiated (A) and differentiated (C) 3T3-L1 cells stably expressing IRAP-EYFP and myc₇-Glut4-ECFP were stained with syntaxin 6 monoclonal antibody and Cy3-conjugated donkey anti-mouse IgG. B and D, undifferentiated (B) and differentiated (D) 3T3-L1 cells stably expressing IRAP-EYFP were treated with 100 nm insulin or carrier (5 μm HCl) for 15 min at 37 °C and analyzed by fluorescence microscopy.