FIGURE 5.

Interaction of IRAP and Glut4 in the yeast two-hybrid system. The lumenal domain of IRAP interacts with the first lumenal loop of Glut4 in yeast two-hybrid system. Panels A and B show negative and positive controls for single transformed cells. Panels C and D show negative and positive controls for double-transformed cells. Panel E shows that double-transformed cells grow on the permissive media (left) on the regular selection media as well as on the high stringency selection media (two middle images) and secret α-galactosidase (blue colonies on the right image).