TABLE 1.
Calorimetric measurements of the binding of fragment 1.2 or fragment 2 to thrombin or prethrombin 2
| Liganda | Titrant | Kd ± S.D. | n ± S.D.b | ΔH ± S.D. | ΔG ± S.D. | ΔS ± S.D. |
|---|---|---|---|---|---|---|
| μm | mol/mol | kcal·mol–1 | kcal·mol–1 | cal·K–1·mol–1 | ||
| P2S195A | F12 | 0.20 ± 0.01 | 0.90 ± 0.01 | –24.2 ± 0.06 | –9.14 ± 0.02 | –51.0 ± 0.2 |
| IIaS195A | F12 | 4.54 ± 0.3 | 0.93 ± 0.02 | –13.6 ± 0.4 | –7.28 ± 0.04 | –21.2 ± 1.2 |
| P2S195A | F2 | 1.59 ± 0.13 | 1.02 ± 0.02 | –11.0 ± 0.25 | –7.90 ± 0.07 | –10.4 ± 1.11 |
| IIaS195A | F2 | 3.14 ± 0.12 | 1.20 ± 0.01 | –11.4 ± 0.1 | –7.50 ± 0.02 | –13.0 ± 0.4 |
a
The species in the cell is denoted as ligand, and the species in the syringe is denoted as titrant
b
Mol of titrant bound/mol of ligand at saturation