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Chitin synthase activity in digitonin-permeabilized cells of different chs mutants
Activity was measured as described previously 23.
| Strain | Activitya |
|---|---|
| YPH499 (WT) | 2132 |
| ECY46-1-8D (chs1Δ) | 30 |
| YMS11 (chs2Δ) | 2311 |
| ECY46-4-1B (chs3Δ) | 4931 |
| Control (blank) | 38 |
Counts/min incorporated in 30 min with [14C]UDP-GlcNAc as substrate.
