FIGURE 6.

Assignment of the cytoplasmic C terminus of CFTR. a, gallery of negatively stained CFTR·anti-FLAG-antibody complexes. Schematic diagrams of CFTR (open particles) and antibodies (filled in gray) are presented below. The location of the cytoplasmic C terminus was assigned to the larger end of the CFTR molecule. CFTR decorated with two FLAG antibodies was frequently observed (right end column). b, gallery of CFTR·Fab-gold complexes. Fab fragments of anti-FLAG antibodies were conjugated with colloidal gold and then mixed with purified CFTR. Similar to the observation in a, the gold conjugate binds to the periphery of the larger domain. CFTR particles bearing two gold particles were also observed (right end column). The scale bars represent 100 Å.