TABLE 2.
Longitudinal diffusion coefficients of EGFP, EGFP-Gαt and rhodopsin-EGFP
| Sample | Treatment | Da | n |
|---|---|---|---|
| μm2/s | |||
| ROS with EGFP-Gαt (f < 1%) | Gα-GTP (glucose only) | 0.016 ± 0.005 | 8 |
| Gα-GTP (α-toxin only) | 0.017 ± 0.005 | 3 | |
| Gα-GTPγS (α-toxin/ATP/GTPγS) | 0.019 ± 0.001 | 4 | |
| R*-Gαβγ (2-DG/sodium azide) | 0.007 ± 0.001 | 8 | |
| Gαβγ-GDP (hydroxylamine) | 0.039 ± 0.005 | 10 | |
| Gα-GTP/no rafts (α-toxin/MβCD) | 0.056 ± 0.005 | 5 | |
| Gα-GTP/+cholesterol (α-toxin/MβCD/cholesterol) | 0.013 ± 0.003 | 3 | |
| Gαβγ-GDP/no rafts (α-toxin/MβCD/hydroxylamine) | 0.037 ± 0.007 | 3 | |
| ROS with EGFP (f < 1%) | Free EGFP | 3.3 ± 0.6 | 6 |
Mean ± S.E.