FIG. 2.

Sitamaquine sensitivity and uptake in different Leishmania species. (A) Differences in sensitivity to sitamaquine between Leishmania species, compared with L. donovani 82, as assessed by Alamar Blue. (B) Differences in sitamaquine uptake between Leishmania species, compared with L. donovani L82, using 5 μM [¹⁴C]sitamaquine for 15 min at 28°C and then washed with 100 μM nonradioactive sitamaquine as described in Materials and Methods. Statistical significance using Student's t test was considered for P values of <0.05. Values for L. donovani L82 versus those of L. mexicana M9012, L. braziliensis LH2419, and L. tropica LRC were significantly different (P < 0.02, P < 0.0005, and P < 0.0003, respectively). Values for L. infantum BCN99 versus L. mexicana M9012, L. braziliensis LH2419, and L. tropica LRC were significantly different (P < 0.04, P < 0.0006, and P < 0.0001, respectively). Values for L. mexicana M9012 versus L. braziliensis LH2419 and L. tropica LRC were significantly different (P < 0.0005 and P < 0.00007, respectively). Data are the means ± standard deviations of five independent experiments. SQ, sitamaquine.