FIG. 2.

Changes in the intracellular pH of yeast cells caused by glucose and fluconazole addition. Yeast cells suspended in a buffer at pH 4.0 were starved of glucose and loaded with CFDA-SE. Cells were then suspended in buffer, pH 4.5, and treated with glucose and fluconazole. Samples were collected at time intervals, and fluorescence at excitation of 435 and 495 nm (F₄₉₅ and F₄₃₅) was measured at 525 nm. F₄₉₅ and F₄₃₅ ratios were used to calculate the intracellular pH values.