TABLE 5.
Initial rates of oligopeptide uptake by yeast mutants
| Oligopeptide | Initial transport rate (nmol/min/mg dry wt) ± SD ofa:
|
|||
|---|---|---|---|---|
| AD | ADCDR1 | ADCDR2 | ADMDR1 | |
| Ala-Ala | 2.55 ± 0.38b | 4.20 ± 0.47b | 3.94 ± 0.50b | 2.61 ± 0.35b |
| 2.58 ± 0.32 | 4.46 ± 0.51 | 4.08 ± 0.44 | 2.59 ± 0.33 | |
| Ala-Ala-Ala | 3.15 ± 0.45 | 5.30 ± 0.57 | 4.74 ± 0.60 | 2.91 ± 0.25 |
| OLys-Leu-Gly (2) | 0.70 ± 0.07b | 1.32 ± 0.06b | 1.26 ± 0.08b | 0.85 ± 0.08b |
| 0.69 ± 0.08 | 1.42 ± 0.09 | 1.31 ± 0.10 | 0.87 ± 0.06 | |
| OLys-Leu-Leu-Gly (3) | 0.51 ± 0.18 | 0.83 ± 0.16 | 0.96 ± 0.12 | 0.65 ± 0.08 |
| OLys-Leu-Leu-Leu-Gly (4) | 0.31 ± 0.12 | 0.49 ± 0.13 | 0.51 ± 0.11 | 0.28 ± 0.08 |
| Leu-FPhe (5) | 0.79 ± 0.08b | 1.82 ± 0.04b | 2.23 ± 0.12b | 0.87 ± 0.11b |
| 0.79 ± 0.07 | 1.95 ± 0.11 | 2.37 ± 0.21 | 0.84 ± 0.09 | |
| Met-Met-FPhe (6) | 0.64 ± 0.09 | 1.11 ± 0.14 | 1.23 ± 0.16 | 0.67 ± 0.10 |
| Nva-FMDP (7) | 0.96 ± 0.09 | 1.86 ± 0.11 | 1.56 ± 0.13 | 1.02 ± 0.10 |
| Lys-Nva-FMDP (8) | 0.79 ± 0.05 | 1.61 ± 0.12 | 1.18 ± 0.09 | 0.81 ± 0.09 |
| Nikkomycin (9) | 0.32 ± 0.02b | 0.98 ± 0.03b | 0.88 ± 0.06b | 0.34 ± 0.04b |
| 0.33 ± 0.05 | 1.08 ± 0.08 | 0.97 ± 0.08 | 0.32 ± 0.05 | |
a
Initial uptake rates were determined as described in Materials and Methods. Values are the means of three determinations ± the standard deviation (SD).
b
Cells were pretreated with fluconazole for 10 min.