FIG. 4.
Diagram showing genome structure of DNA insert in fosmid clone T45. Sequence analysis and editing were carried out using the program Artemis (http://www.sanger.ac.uk/Software/Artemis/), and the locations of genes were predicted using the heuristic approach for prokaryotic-gene predictions under the program GeneMark (http://exon.biology.gatech.edu/GeneMark/). Numbers inside arrows represent orfs in T45. Amino acid sequence identities to equivalent ORFs in the VanG operon in E. faecalis (shown in gray) are shown underneath the arrows. Tandem Tcr genes are highlighted in black. The locations of primer pairs used for assessing the presence of the transposon-like element and tandem Tcr genes are shown. BlastP results for all ORFs are summarized in Table S1 in the supplemental material. The gap in the sequence between orfs 20 and 21 is indicated (//).