FIG. 5.
PCR screening of new mobile element carrying the tandem Tcr genes in other clones in the metagenomic library (T2 to T21), C. saccharolyticum K10, and a representative R. inulinivorans-K10 transconjugant (Tc) genomic DNA. Primers were designed from the sequence of T45 in such a way that the PCR products were contiguous along the length of the clone, as shown in Fig. 4. The sizes of the PCR products were predicted to be 2 kb in all cases, except for tet(O/32/O) and tet(40), which were approximately 2.5 kb each (marked by asterisk). The gap in the sequence of fosmid clone T45 between primer pairs 10 and 11 is indicated (//).