TABLE 1.
Procedure | Primer name | Primer sequence | Reference or source |
---|---|---|---|
Genome walking | Tet32-3′ | TCATTCTGAAAGGAGAAATCCCTGCTAG | This work |
TetExt | ACCGCAGGAATATCTCTCACGGGCGTA | This work | |
Tcr gene amplification | TetOFF2 | TTGTTTTGGGGCTATTGGAG | 26 |
TetOFR3 | TATATGACTTTTGCAAGCTG | 26 | |
TetWarray-for | GGAGGAAAATACCGACATA | 27 | |
TetWarray-rev | AATCTTACAGTCCGTTACG | 27 | |
Tet32(2)array-rev | CTCTTTCATAGCCAGGCC | 27 | |
TetQarray-for | CAAGATGTCCTGTTTATGC | 27 | |
TetQarray-rev | GAATCCCTTCAAAAACGC | 27 | |
Sequencing | 519r | GWATTACCGCGGCKGCTG | |
Fosmid subcloning | adapt-I | AATTCGGCACGAGG | This work |
adapt-IIa | O3P-CCTCGTGCCG | This work |
This primer is phosphorylated at the 5′ end.