TABLE 2.
Effects of tetracycline with or without CCCP on growth of E. coli cells containing tet(40) or tet(W)
| Gene | Tetracycline concn (μg/ml) | Avg (±SD) OD650a
|
% Growthb | |
|---|---|---|---|---|
| Plus tetracycline | Plus tetracycline and CCCP | |||
| tet(40) | 40 | 0.47 ± 0.04 | 0.26 ± 0.03 | 56.5 |
| 60 | 0.33 ± 0.07 | 0.17 ± 0.03 | 53.4 | |
| 80 | 0.20 ± 0.005 | 0.03 ± 0.004 | 16.5 | |
| tet(W) | 40 | 0.91 ± 0.04 | 0.87 ± 0.02 | 96.4 |
| 60 | 0.80 ± 0.09 | 0.79 ± 0.09 | 99.2 | |
| 80 | 0.73 ± 0.06 | 0.55 ± 0.03 | 75.4 | |
a
The OD650 measurements given are the averages (±standard deviations) from triplicate tubes for a single experiment, except those quoted in the presence of 60 μg/ml tetracycline, which are the averages from triplicate tubes for three separate experiments.
b
Percentage of growth in the presence of CCCP and tetracycline compared to that in the presence of tetracycline alone.