TABLE 1.

MICs of β-lactams for C. freundii KHM243, E. coli W1895(pCF243) transconjugant, E. coli JM109(pKHM-1) expressing the KHM-1 MBL, and E. coli host strains

Antibiotic(s)a	MIC (μg/ml)
	C. freundii KHM243	E. coli W1895 (pCF243)b	E. coli W1895	E. coli JM109 (pKHM-1)c	E. coli JM109
Ampicillin	256	64	8	16	1
Ampicillin-sulbactam	64	64	1	16	0.5
Ticarcillin	>512	>512	2	512	2
Ticarcillin-clavulanic acid	512	>512	4	512	2
Piperacillin	4	16	2	4	0.25
Cephaloridine	512	128	2	64	1
Cefuroxime	>512	>512	2	>512	8
Ceftazidime	>512	>512	0.125	>512	0.063
Cefotaxime	64	>512	0.008	128	0.004
Cefepime	32	>512	0.002	64	0.004
Cefozopran	16	256	0.016	64	0.008
Imipenem	2	4	0.063	0.5	0.063
Meropenem	4	4	0.004	4	0.004
Aztreonam	0.25	0.063	0.031	0.063	0.031
Carumonam	0.25	0.125	0.031	0.063	0.031
Cefoxitin	512	>512	8	>512	8
Cefmetazole	512	512	0.5	>512	0.25
Cefotetan	128	512	0.125	>512	0.031
Cefbuperazone	128	256	0.063	512	0.031
Cefminox	512	>512	0.125	512	0.25
Moxalactam	256	>512	0.063	>512	0.031
Flomoxef	64	256	0.031	128	0.031

^aThe ratio of the ampicillin to sulbactam was 2:1. The ratio of ticarcillin to clavulanic acid was 15:1.

^bNatural plasmid carrying the bla _KHM-1 gene.

^cRecombinant plasmid constructed by insertion of DNA fragment containing the bla_KHM-1 gene into the cloning vector pHSG396.