TABLE 2.

Isolates with ESBL genes

Speciesa	Isolate (JIE)b	Drug resistance profilec	blaTEM variantd	blaSHV variant	Inc groupe
Isolates with blaCTX-M-15 (n = 33)
E. coli B2	101	CTX CAZ GENTOBSXT CIP	1b		FIA+FIB+FII+N
E. coli B2	118,157,224	CTXCAZGENTOBSXT CIP	−, 1b, 1b		FIA+FII
E. coli B2	186/295	CTXCAZGENTOB SXT CIP	1b/1b		FII
E. coli B2	250	CTXCAZGENTOB FOX SXT CIP	1b		FIA+FIB+FII
E. coli D	085,166,204	CTXCAZGENTOB FOX SXT CIP	1b, −, −		FIA+FIB+FII
E. coli B2	106, 110	CTX CAZ GEN TOB SXT	1a, 1b		−
E. coli D	236/242	CTXCAZGEN TOB SXT	1b/1b		I1
E. coli D	174	CTX CAZ GEN TOB CIP	1b		I1
E. coli A	189, 291	CTX CAZ GEN TOB CIP	−, 1i		−
E. coli B2	188	CTX CAZ GEN TOB CIP	1b		−
E. coli B2	100	CTXCAZ CIP	1b		FII
E. coli B2	143	CTXCAZ CIP	1b		ND
E. coli A	222	CTX CAZ CIP	1b		−
E. coli B2	097/154/286	CTXTOBSXT CIP	1b/−/−		FIA+FII
E. coli D	134	CTXCAZGENTOB FOX CIP			FIA+FIB+FII
E. coli D	098	CTXCAZGEN TOB FOX CIP	1i		FIA+FIB+FII
E. coli B1	113	CTXCAZ SXT CIP	1b		I1
E. coli D	139	CTXCAZ SXT CIP	1b		I1
E. coli B2	289	CTX GEN TOB CIP			−
K. pneumoniae	120/127,146	CTXCAZGENTOBSXT CIP	1b (all)	11 (all)	ND
K. pneumoniae	162f	CTX CAZ GEN TOB FOX SXT CIP	1b	11, 12	−
Isolates with blaCTX-M-3 (n = 4)
E. coli D	161	CTX GEN TOB FOX SXT CIP	1b		FII+B
E. coli D	095	CTX GEN TOB FOX SXT	1b		−
E. coli D	077	CTX GEN FOX SXT CIP	1b		N
E. coli B2	251	CTX GEN CIP	1b		FII+Y
Isolate with blaCTX-M-62 (n = 1)
K. pneumoniae	137	CAZSXT	1b	1	ND
Isolates with blaCTX-M-14 (n = 17)
E. coli A	081	CTX GEN TOB SXT	1b		FII
E. coli D	084g, 110b, 196	CTX GEN TOB SXT	1b, 1b, 1i		FII
E. coli D	182	CTX GEN TOB SXT	1b		B
E. coli D	153, 180	CTX SXT CIP	−, 1b		FII
E. coli D	201	CTX GEN TOB FOX SXT CIP	1b		K
E. coli D	168	CTX GEN TOB SXT CIP	1b		ND
E. coli D	121	CTX GEN TOB FOX CIP	1b		−
E. coli A	088	CTX GEN CIP	1b		I1
E. coli B2	052	CTX CAZ GEN TOB SXT	1b		B
K. pneumoniae	014/021/025/056	CTXGEN TOB SXT	1b (all)	11 (all)	ND
K. pneumoniae	223	CTX		11	FII
Isolates with blaCTX-M-9 (n = 3)
E. coli D	059/084g/277	CTX GEN TOBSXT	−/1b/−		FIB
Isolates with blaCTX-M-24 (n = 2)
E. coli D	216	CTX GEN TOB FOX SXT CIP	1b		FII
E. coli B2	298	CTX			FII
Isolate with blaCTX-M-27 (n = 1)
E. coli A	058	CTXCAZGEN TOB FOX SXT CIP	1b		FII
Isolates with blaSHV-12 as the only ESBL gene (n = 6)
E. coli D	163	CAZSXT	1b	12	FIB
E. coli B2	038	CAZ SXT	1b	12	−
E. coli D	119	CTX CAZ GEN TOB FOX SXT CIP	1b	12	−
E. coli B2	124	CAZGENTOBSXT		12	A/C
K. pneumoniae	024	CTX CAZ GEN TOB FOX SXT CIP	1b	1, 12	−
K. pneumoniae	205	CTX CAZ GEN TOB FOX SXT CIP	1b	11, 12	−

^aE. coli phylogenetic groups are shown.

^bJIE isolates (shown in the table without JIE prefix) with identical DNA fingerprints (pulsed-field gel electrophoresis) are separated by slashes (e.g., 097/154/286), and JIE isolates with dissimilar DNA fingerprints are separated by commas. JIE isolates from which the ESBL gene was transferred to E. coli by conjugation are underlined. −, absence of bla_TEM gene.

^cIsolates were not susceptible by NCCLS/CLSI guidelines (20) to drugs unless left blank. None were resistant to imipenem or amikacin. Resistance phenotypes transferred to E. coli by conjugation are underlined. CTX, cefotaxime; CAZ, ceftazidime; GEN, gentamicin; TOB, tobramycin; FOX, cefoxitin; SXT, trimethoprim-sulfamethoxazole; CIP, ciprofloxacin.

^dbla_TEM-1i is identical to bla_TEM in GenBank accession no. EF035590 (E. coli, India), a C228T variant of bla_TEM-1a (e.g., EMBL accession no. X54604); slashes and commas reflect identity relationships as in footnote a above.

^eInc group, incompatibility groups of conjugative plasmids; ND, Inc group not determined; −, no plasmid transferred.

^fNeither bla_CTX-M-15 nor bla_SHV-12 was transferred from isolate JIE162 by conjugation.

^gThe JIE084 isolate carried both bla_CTX-M-9 and bla_CTX-M-14; only bla_CTX-M-14 was found in the IncFII-positive transconjugant.