FIG. 2.

Growth tests of HydS and HydR3 parental strains (A and C) and transformants (B and D) on fenhexamid. (A) HydR3⁺ strains compared to the B05.10 reference strain. (B) B05.10 transformants harboring the indicated allele on the pFTELerg plasmid. (C) HydR3⁻ strains. (D) B05.10 transformants harboring the indicated allele on the pFTELerg plasmid. TR5.10, transformants carrying the empty pFACR5 vector (see Materials and Methods). Growth was monitored on YSS medium with the indicated fenhexamid concentrations after 7 days at 21°C. The growth medium was supplemented with hygromycin B at 50 μg ml⁻¹ for testing growth of the transformants (B and D). All strains and transformants are in bold; the erg27 alleles are in italics.