TABLE 2.
Oligonucleotides used in this studya
| Name | 5′ end | Strand | Sequence (5′-3′) |
|---|---|---|---|
| erg27LP2-Xho | 490 | D | AGTCTCGAGATGGAGCGGCAGCGGGTAAT |
| erg27Beg | 1261 | D | TGGGATTACCACCATGGGAGACAAGTG |
| erg1800down | 1764 | D | CCGCCACTTATTCCGCAGATGTT |
| erg2600down | 2554 | D | TGTAAGATGGATGGGAAGCCAATG |
| erg1900up | 1906 | R | CCCTGCATTCAAGACTACAACATCCAG |
| erg2000up | 2067 | R | TCGGAGGGTTTGGCTTGTTTTG |
| erg2600up | 2670 | R | GAAGCTGCCCCGTCCATGTTATC |
| erg3200up | 3295 | R | GCTTGGGCTACTTTAGATGTGA |
| erg27End | 2789 | R | CAATGGTTCCGCATTTCTTTGCCTCCC |
| erg27RP-Bam | 3408 | R | AGGATCCGCACGAGGCGTGCCTAACTCA |
a
B. cinerea specific sequences are in bold; adapter sequences are in lightface. The positions of the 5′ ends are indicated relative to the erg27 gene presented in Fig. 1. D, direct; R, reverse.