FIG. 4.

Relative expression of Cdr1p and Cdr2p in replicate Western blots of PAGE-separated whole-cell extracts from C. albicans clinical isolates. (A) Cell extracts were prepared as described in Materials and Methods and separated by PAGE. Each lane was loaded with an equivalent amount of total protein. Replicate blots were incubated with anti-Cdr1p antibodies (upper row) anti-Cdr2p antibodies (center row) or anti-Mdr1p (lower row) at dilutions that gave equivalent band intensities with control recombinant Cdr1p, Cdr2p or Mdr1p plasma membrane preparations run on the same gels. The control bands shown are of representative blots of AD/CDR1 (upper band) AD/CDR2 (center band) or AD/MDR1 (lower band). FLC MICs for each strain are indicated. (B) Relationships between FLC MICs for 18 C. albicans strains and Western blot band intensities obtained with either anti-Cdr1p or anti-Cdr2p antibodies. Cdrp band intensities on the blots of cell extracts, incubated with either anti-Cdr1p antibodies or anti-Cdr2p antibodies, were measured by image analysis as described in Materials and Methods. Band intensity values plotted have been corrected using the appropriate recombinant control band intensity measured on the same blot.