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. 2008 Aug 27;82(21):10792–10802. doi: 10.1128/JVI.00903-08

FIG. 7.

FIG. 7.

Knockdown of endogenous CAPERα promotes colony formation by v-Rel-transformed CSCs. (A) RT-PCR showing efficacy of siRNA 233-mediated knockdown of endogenous chicken CAPERα at 72 h after electroporation of v-Rel-transformed CSCs, compared a nonspecific control siRNA (NC 1). Chicken GAPDH (glyceraldehyde-3-phosphate dehydrogenase) mRNA levels were analyzed as a control. (B) Effect of siRNA-mediated knockdown of endogenous chicken CAPERα or the NC 1 siRNA control on the ability of v-Rel-transformed CSCs or the control ALV-transformed DT40 chicken pre-B cell line to form colonies in soft agar. The averages from three independent experiments are shown with standard deviations.