FIG. 2.

Dominant-negative Dyn2(K44A) expressed in virus-producing cells selectively inhibits the effect of Nef on the single-round infectivity of Env-deficient virions complemented with HIV-1 Env in trans (left) and of CD4-chemokine receptor pseudotypes (right). Pseudotypes were produced in the presence of a HIV-1 vector encoding GFP and used to infect TZM-bl cells or HeLa cells transiently expressing Env_HXBΔCT, as appropriate. Infectivities were evaluated by counting GFP-positive foci. Error bars indicate standard deviations.