FIG. 3.
Clonotype frequency of two p7756-770 peptide-specific (AASLAGTHGLVSFL; restricted by Patr A*0701) and dominant TCR clonotypes measured using real-time PCR assay of PBMC (solid lines) and liver biopsy samples (dotted lines) of HCV-infected chimpanzee (CB0556). Each point indicates the mean of triplicate values and its standard error. Open symbols indicate undetectable levels of the specific clonotype, and the minimal detectable frequency was calculated by using the lowest limit of detection on the standard curve as the nominator. The sensitivity of detection of each clonotype was at least 10 copies as measured by using molecularly cloned TCRs. The three vertical lines indicate the administration of three doses of anti-CD8 antibody. Week 0 indicates the times of HCV infection. GE, genome equivalents.