FIG. 3.

Functional HPV-16 E1 can be expressed from a promoter other than P97. Replication in SCC13 cells transiently transfected with equimolar quantities of complementary HPV-16 W12 constructs was assayed by Southern blotting as previously described. Mutations in the HPV-16 ORFs and cis elements are illustrated in Fig. 1. E1−, E1 ORF; E8−, E8 ORF; T(65) and T(7890), TATAA boxes at nucleotide positions 65 and 7890; Sp1#1−, mutation of Sp1#1 site. Linearized HPV-16 genome (30 pg) was included as a positive blotting control.