FIG. 3.

Frequencies of HIV-specific tetramer-positive cells among ex vivo expanded cells. CSFMCs and PBMCs were expanded ex vivo for 2 weeks with PHA and then assessed for frequencies of HIV-specific tetramer-positive CD8⁺ cells. (A) Representative flow cytometric plots show tetramer frequencies among unexpanded and expanded cells from CSF. (B) Relationship between tetramer frequencies among ex vivo expanded CD8⁺ cells from CSF and freshly isolated cells from CSF. (C) Relationship between tetramer frequencies among ex vivo expanded CD8⁺ cells from CSF and ex vivo expanded CD8⁺ cells from blood. The diagonal dashed line represents the line of unity. The P value is derived from a two-tailed Wilcoxon matched-pairs t test.