FIG. 1.

Baculovirus-expressed UL16-GFP interacts with UL11 via the AC and LI motifs. (A) Diagrams of GST-UL11 constructs. Approximately equal amounts of these constructs as estimated from a Ponceau S-stained gel (B) were mixed with lysates from uninfected (M, mock) or BV.UL16-GFP-infected Sf21 insect cells. The UL11 AC mutant has the approximately same mobility as the Δ51-96 mutant, which has been observed previously (14, 15). (C) Proteins bound to GST constructs were loaded onto SDS-PAGE gels and analyzed by immunoblotting for GFP. When analyzed using densitometry, approximately 40% of the amount of UL16-GFP protein in the lysates (input) was pulled down by GST-UL11.