FIG. 4.

UL11 cannot interact with NEM-modified UL16 from infected cell lysates. (A and B) Vero cells were infected with HSV.UL16-CFP at an MOI of 1. At 20 h postinfection, cells were scraped into PBS. One set of cells were treated with NEM for 30 min before detergent lysis. Cell lysates were incubated with approximately equal amounts of glutathione bead-bound GST-UL11 as estimated from a Ponceau S-stained gel (A). Bound proteins were analyzed by SDS-PAGE and immunoblotting (B). For lanes 2 and 3, the addition of NEM was either after (A) or prior (P) to the 2-h incubation. (C and D) GST-UL11 fusion proteins as detected in a Ponceau S-stained gel (C) were mixed with either untreated or NEM-treated HSV.UL16-CFP- infected cell lysates, and bound proteins were analyzed by SDS-PAGE and immunoblotting.