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. 2008 Aug 27;82(21):10567–10579. doi: 10.1128/JVI.01308-08

FIG. 3.

FIG. 3.

Ad vectors with increased affinity to CD46. (A) Analysis of vector genomes. (Top) Structure vector genomes. (Bottom) Restriction endonuclease analysis (X, XhoI; H, HindIII) of vectors genomes isolated from purified Add5/35, Ad5/35+, and Ad5/35++ particles. The distinguishing DNA fragments are, for Ad5/35+, the 3.14- and 1.78-kb XhoI fragments and, for Ad5/35++, the 2.53- and 2.14-kb HindIII fragments. M, 1-kb ladder marker. wt, wild type; CMV, cytomegalovirus; ITR, inverted terminal repeat. (B) Biacore analysis of Ad-sCD46 interactions. Purified Ad particles were immobilized, and binding to sCD46 was analyzed. Sensorgrams are shown for injections at seven different concentrations of sCD46 (50 to 0.781 μg/ml in a twofold dilution series). The analysis was performed in triplicate. KD, equilibrium dissociation constant; ka, association rate constant; kd, dissociation rate constant.