FIG. 7.

Analysis of CD46 distribution in infected MO7e cells. (A) Colocalization of CD46 and virus. CD46 (green) was analyzed before infection and at 15 min and 4 h after infection with Ad5/35 and Ad5/35++ at an MOI of 4,000 VP per cell. Cathepsin B-positive endosomal compartments are green. Confocal images of cells were taken in both green and red channels. Representative fields are shown. (B) Analysis of CD46 on the surfaces of MO7e cells and GFP expression at different time points after infection with Ad5/35, Ad5/35+, or Ad5/35++ at MOIs of 50 PFU/cell and 400 PFU/cell. Notably, staining for the viral hexon at 8 h postinfection did not reveal any signal at the cell surface, indicating that the decrease in CD46-PE fluorescence was not due to competition with Ad particles bound to surface CD46. Shown are the averages of three independent experiments. The standard deviations were less then 10% from the averages for all data points.