FIG. 8.

GFP expression in organs and liver metastases after intravenous injection of Ad5/35 and Ad5/35++ into CD46-transgenic mice bearing CD46^high liver metastases. Mice were intraportally injected with TC1-CD46 tumor cells. Three weeks later, when liver metastases were established, mice received tail vein injection of 3 × 10⁹ PFU of Ad5/35 and Ad5/35++ (n = 4). Organ sections were analyzed for GFP expression 3 days later. (A) Representative sections. (B) Quantification of GFP-positive cells/mm² in liver metastases (tumor), liver, and spleen (20 sections per mouse, four mice per virus). (C) Quantification of GFP-positive cells in cell suspensions of microdissected liver metastases. Tumor tissue was digested with collagenase and versene, and cell suspensions were analyzed by flow cytometry for GFP and CD46 expression. Representative samples are shown. Transduced TC1-CD46 cells appear as GFP⁺ CD46^high cells (upper right quadrant). Cells in the upper and lower left quadrants represent cell debris that stained positive with propidium iodide (data not shown).