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. 2008 Sep 3;82(21):10502–10509. doi: 10.1128/JVI.00970-08

FIG. 6.

FIG. 6.

Growth enhancement of a PR8/H5N1 reassortant mediated by NA and NS genes. (A) MDCK cells were infected with reassortant viruses containing mHA from either VN1194 or VN1203 and NA from a different N1 virus or NS from PR8(Cambridge) at an MOI of 0.01. Virus titers were determined in a plaque assay with MDCK cells at 36 hpi. The data are reported as mean titers with standard deviations for three independent experiments. Titers significantly (P < 0.05) increased (*) compared with that of the PR8(UW)/VN1194H5N1 virus are shown and compared with those of PR8(UW)/VN1203H5/VN1203FillN1 and PR8(UW)/VN1203H5/HK213N1 (**). (B) MDCK cells were infected with reassortant viruses containing H5 mHA from clade 1 and clade 2 strains with homologous NA or HK213 NA or PR8(Cambridge) NS instead of PR8(UW) NS at an MOI of 0.01. Virus titers were determined in a plaque assay with MDCK cells at 36 hpi. The data are reported as mean titers with standard deviations for three independent experiments. Titers significantly (P < 0.05) increased compared with those of the viruses possessing homologous NA (*) or compared with those of viruses possessing HK213 NA (**) are shown.