FIG. 4.

Axonal transport of capsids and glycoproteins in human neurons infected with the HSV gI⁻ mutant. SK-N-SH neurons were infected with the repaired F-gI/GFP-R (A) or the gI⁻ mutant F-gI/GFP (B) for 18 h, fixed, permeabilized, and stained with mouse anti-VP5 MAb and rabbit anti-gB polyclonal antibodies, followed by Texas red-conjugated donkey anti-mouse and Cy-5-conjugated anti-rabbit secondary antibodies. Scale bars, 5 μm. (C and D) Quantification of VP5 puncta (C) and gB puncta (D) in different segments of neuronal axons measured from the neuronal-cell body. VP5 or gB puncta were counted in 13 F-gI/GFP-infected and 10 F-gI/GFP-R-infected neurons involving four separate experiments. Each symbol represents the number of puncta present in an individual axonal segment. Statistically significant values are shown as asterisks, with P < 0.05 marked as two asterisks and P < 0.001 marked as three asterisks.