FIG. 5.

Axonal transport of capsids and membrane glycoproteins in human neurons infected with an HSV US9⁻ mutant. Differentiated SK-N-SH neurons were infected with either the repaired F-US9/GFP-R (A and C) or the US9-null mutant F-US9/GFP (B and D) for 18 h, fixed, permeabilized, and stained with mouse anti-VP5 MAb and rabbit anti-gD polyclonal antibodies (A and B) or mouse anti-VP5 MAb and rabbit anti-gB polyclonal antibodies (C and D), followed by Texas red-conjugated donkey anti-mouse and Cy-5-conjugated anti-rabbit secondary antibodies. Scale bars, 5 μm. (E and F) Quantification of VP5 puncta (E) and gB puncta (F) in axonal segments measured from the neuronal-cell body. Puncta were counted in the axons of 13 F-US9/GFP-infected and 13 F-US9/GFP-R-infected neurons involving four independent experiments. Each symbol represents the number of puncta present in an individual axonal segment. Statistically significant values are shown as asterisks, with a P value of <0.05 marked as two asterisks and a P value of <0.001 marked as three asterisks.