FIG. 6.

gE does not associate with capsids in axons of HSV-infected neurons. SK-N-SH neurons were infected with F-VP26-GFP, which expresses a fluorescent capsid protein for 24 h, and then the neurons were fixed, permeabilized, and stained with rat anti-gE/gI polyclonal antibodies, followed by Texas red-conjugated donkey anti-mouse secondary antibodies. Representative results from four independent experiments are shown. (A) Rat anti-gE/gI staining. (B) VP26-GFP fusion protein fluorescence. (C) Merged fluorescence. (C1 and C2) Higher magnifications of the areas boxed in panel C. Scale bars, 5 μm (A to C) and 1 μm (C1 and C2).