FIG. 8.

US9-specific antibodies stain capsid puncta in axons of HSV-infected neurons. SK-N-SH neurons were infected with wild-type HSV for 18 h, fixed, permeabilized, and stained with mouse anti-VP5 MAb and simultaneously with affinity-purified rabbit anti-US9 antibodies, followed by Texas red-conjugated donkey anti-mouse secondary antibodies and fluorescein isothiocyanate-conjugated donkey anti-rabbit secondary antibodies. Panels A to C are representative of three independent experiments. (A) Mouse anti-VP5 staining. (B) Rabbit anti-US9 staining. (C) Merged fluorescence. (C1 and C2) Higher magnifications of the areas boxed in panel C. Scale bars, 5 μm (A to C) and 1 μm (C1 and C2).