Figure 6.

Expression of GFP-ΔBLCBS-myosin in comparison to that of myosin and GFP-myosin. (A–C) Whole cell lysates were run on 7.5% SDS-PAGE gels. Lane 1, myosin null cells; lane 2, cells expressing GFP-ΔBLCBS-myosin; lane 3, cells expressing GFP-myosin; lane 4, cells expressing wild-type myosin. (A) Coomassie-stained gel. (B) Immunoblot probed with antimyosin antibody. (C) Immunoblot probed with anti-GFP antibody. (D) Neither light chain binds to GFP-ΔBLCBS-myosin. Purified GFP-ΔBLCBS-myosin protein and wild-type myosin were run on a 15% SDS-PAGE gel and subsequently Coomassie stained to visualize the light chains. MHC, myosin heavy chain; ELC, essential light chain; RLC, regulatory light chain.