FIG. 1.

Schematic diagram of the Con1 subgenomic replicons used to measure transient RNA replication efficiency in the Huh-7.5 cell line. The Con1 wild-type replicon (C1/WT) is shown at the top with the 5′ and 3′ NTR structures illustrated. The truncated C1/WT open reading frame, lacking the E1-p7 coding region, is depicted as a shaded box with the polyprotein cleavage products indicated along with the position of the cell culture-adaptive mutation S2204I in NS5A. Shown below are H77 NS4B and hybrid Con1-H77 NS4B proteins that replace Con1 NS4B in chimeric replicons. The adopted nomenclature for each subgenomic replicon is indicated on the left and the three NS4B domains, the N terminus (residues 1 to 47), the central membrane-spanning region (residues 48 to 227), and the 34 C-terminal amino acids (residues 228 to 261) are shown. Shaded regions represent Con1 NS4B sequences (C1) and open boxes denote H77-derived NS4B sequences (H).