TABLE 1.
Oligonucleotides used for constructing chimeric and mutant Con1 repliconsa
| Name | Sequence (5′ → 3′) |
|---|---|
| 41 | (+) CATCATGGCATGCATGTCGG |
| 46 | (−) CCACGGATCCGGAGCATGGCGTGGAGC |
| 48 | (−) GTGGTGTACGCGTTAATGGG |
| 69 | (−) CCATCTGCATTCCCTGTTCGATGTAAGGGAGGTGTTGGGAGCACTCTTCCATCTCATCGA |
| 76 | (−) GAGCTGCTCAGCGAGCATCATCCCTTGCTCGATGTACGGTAAGTGCTGAGAGCACTCTTCCATCTCATCGA |
| 77 | (+) TCATGGCATGCATGTCGGCT |
| 99 | (+) CGACCCTCGAGGCCTTCTGGGCGAAGCATAT |
| 112 | (−) ACACGTGCGGCCGCGTCGCTCTCAGGCACATAGT (creates a NotI site) |
| 113 | (+) GCGATGCGGCCGCCCGCGTCACTGCCATACTCAG (creates a NotI site) |
| 115 | (+) GCGACGCGGCCGCACGTGTCACTCAGATCCTCTC (creates a NotI site) |
| 148 | (−) GCTTGGACTGGAGCCAGGTC |
| 185 | (−) CCAAGGATCCGGAGCATGGCGTGGAGCAGTCCTCGTTGATCCACTGGTGAAGCCTCCTCAGCAGCTGAGTGATGGTAA |
| 186 | (+) CTAAGCGGCCGCCCGCGTCACTGCCATCCTCTCTAGTCTTACCAT (creates a NotI site) |
| 190 | (−) CACACTCGAGGGTCCGCCACTTGGATTCCACCACGGGAGCAGCAGCCTCTGCTTGGCGGGACGCG |
| 237 | (+) GCGACGCGGCCGCACGTGTCACTCAGATCCTCTCTAGTCTTACCGTAACTCAGCTGCTGAAGAGG (creates a NotI site) |
| 238 | (−) CCACGGATCCGGAGCATGGCGTGGTGCACTCCGAGCTGATCCACTGGTGAAGCC |
| 260 | (−) CCACGCGCGCCTGCCACCCCTGCTCCATAACCTGCCAAAATATCCACAAGCACCTTCCCAAGGCCTATGCTGCCGATAGCCGCTCCAGCGATGCC |
| 261 | (−) CCACGCGCGCCTGCCACCCCTGCTCCATAACCTGCCAAAATATCCACAAGCACCTTCCCAAGGCCAACGCTGCCAACAGCCGCTCC |
| 262 | (−) CCACGCGCGCCTGCCACCCCTGCTCCATAACCTGCCAAAATATCCACAAGCACCTTCCCAAGGCCAACGCTGCCGATAGCCGCTCCAGCGATGCC |
a
Restriction sites used for cloning are underlined and the polarity of each oligonucleotide is indicated as either genome RNA (+) or its complement (−).