TABLE 2.
Locations and frequencies of NS3-4A second-site mutations identified in G418-resistant Huh-7.5 clones supporting replication of Con1-H77 NS4B chimeras
| Location | Mutation | No. of indicated clones selecteda
|
|||
|---|---|---|---|---|---|
| C1Neo/H4B | C1Neo/HN47Tm | C1Neo/HN47C34 | C1Neo/HTmC34 | ||
| NS3 protease | Q1112R | 2 | 1 | ND | 1 |
| P1115R | ND | ND | ND | 1 | |
| E1202G | 1 | ND | 1 | 1 | |
| M1205K | ND | 1 | ND | ND | |
| NS3 helicase | A1218D | ND | ND | 1 | ND |
| R1283G | ND | 1 | ND | ND | |
| T1287K | ND | ND | 1 | ND | |
| S1369R | 3 | 1 | 1 | 1 | |
| D1431N | ND | ND | 1 | 2 | |
| D1431Y | 1 | ND | 1 | ND | |
| S1560G | 1 | ND | ND | ND | |
| NS3 protease + helicase | P1115R + S1560G | 1 | ND | ND | ND |
| NS4A | K1691R | ND | ND | 1 | ND |
a
ND, not detected.