TABLE 1.

Overall genotype distribution by clone-based sequencing of the 5′ UTR of HCV after multigenotype HCV challenge^a

Wks postchallenge	Sample	% Genotype distributionb(no. with genotype/total clones)
		1a	1b	2	3	4	5	6
2	#405					78 (14/18)	22 (4/18)
	#409	17 (3/18)	11 (2/18)		72 (13/18)
	#412					100 (18/18)
	#414					100 (20/20)
	Total clones	4.1	2.7		17.6	70.3	5.4
	Total viremia	<0.01	<0.01		<0.02	87.4	12.6
8-10	#405					100 (8/8)
	#409	56 (5/9)	44 (4/9)
	#412	67 (6/9)		22 (2/9)	11 (1/9)
	#414			100 (8/8)
	Total clones	32.4	11.8	29.4	2.9	23.5
	Total viremia	5.3	0.7	92.3	0.7	1.0
36-48	#405				100 (8/8)
	#409	100 (9/9)
	#412		100 (10/10)
	#414	100 (8/8)
	Total clones	48.6	28.6		22.9
	Total viremia	89.4	3.6		6.9
60, 64	#405
	#409
	#412
	#414	100 (5/5)

^a5′ UTRs were cloned and sequenced to determine the genotype distribution from the following samples (weeks after multigenotype HCV challenge): for animal 405 at 2, 8, 48, 60, and 64 weeks; animal 409 at 2, 8, 40, 60, and 64 weeks; animal 412 at 2, 10, 49, 60, and 64 weeks; animal 414 at 2, 8, 36, 60, and 64 weeks. The results from weeks 60 and 64 were the same.

^bThe clonal genotype distribution in each chimpanzee was normalized with the individual viral load to represent actual genotype distributions in the animals. Briefly, viremia titers at the indicated times were multiplied by the clonal genotype distribution, and the main genotype population in the chimpanzee at the indicated times is shown in bold.