FIG. 7.

Cholesterol-depleted DHBV binds and enters into PDHs. CD- or mock-treated DHBV particles were attached to PDHs. Cells were harvested with or without trypsin or warmed to 37°C for 1 h after removal of the inoculum. Both inoculum and cell lysates were subjected to immunoblotting and PCR analysis for viral L protein (A) and DNA (B), respectively. Vimentin served as a loading control. PCR was standardized with serial dilutions of a viremic serum with known GE.