FIG. 2.

The C-terminal 100 amino acids of hUL47 can direct a nuclear protein into the cytoplasm. (A) Line diagram of hUL47 and its homologue from BHV-1, bUL47, showing previously identified NLS, RNA binding (RBD), and NES domains. (B) Line drawing of GFP fusion proteins used in this study. (C) Line drawing of hUL47 and the corresponding seven peptides that were fused to GFP-SV40-NLS. (D) Plasmids expressing the fusion proteins were transfected into Hep-2 cells grown on coverslips. Twenty hours later, the cells were fixed with paraformaldehyde, examined by confocal microscopy, and scored for relative nuclear/cytoplasmic localization.