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The hUL47 NES is a 10-residue hydrophobic peptide. (A) Line drawing of the region of hUL47 covered by peptide 7, together with the smaller peptides used to refine the hUL47 NES. (B) Hep-2 cells grown on coverslips were transfected with plasmids expressing GFP-NLS fused to the regions indicated in panel A. Representative images were acquired 20 h later using confocal microscopy and scored for relative nuclear/cytoplasmic localization.
