TABLE 1.

Immunization and challenge protocol

Immunogen	Challenge, by groupa
	Group 1, DNA	Group 2, DNA/ IL-12	Group 3, DNA/ IL-15	Group 4, control
DNA,b wks 0 and 4, intramuscular	SIVmac239env	SIVmac239env	SIVmac239env	Control DNA
	SIVmac239gag	SIVmac239gag	SIVmac239gag
	SIVmac239rev/nef	SIVmac239rev/nef	SIVmac239rev/nef
	Control DNA	DNA/IL-12	DNA/IL-15
Ad5hr-SIV,c wk 12, intratracheal	SIVsmH4env/rev	SIVsmH4env/rev	SIVsmH4env/rev	Ad5hr-ΔE3 vector
	SIVmac239gag	SIVmac239gag	SIVmac239gag
	SIVmac239nefΔ1-13	SIVmac239nefΔ1-13	SIVmac239nefΔ1-13
Proteins,d wk 24 and 36, intramuscular	SIVmac251 gp140	SIVmac251 gp140	SIVmac251 gp140	MPL-SE
	SIVmac239 Nef	SIVmac239 Nef	SIVmac239 Nef	Adjuvant
Challenge,e wk 48, intrarectal	SIVmac251	SIVmac251	SIVmac251	SIVmac251

^aGroups 1 to 3 had seven macaques per group; group 4 had four macaques.

^bAt 2.5 mg DNA per dose, mixed together and administered at multiple sites in both thighs.

^cAt 5 × 10⁸ PFU/recombinant; total Ad dose was 1.5 × 10⁹ PFU.

^dSIV gp140, 100 μg/dose; SIV Nef, 50 μg/dose.

^eAt 10 50% monkey infectious doses.