FIG. 7.

HCV infection induces increased production of mitochondrial superoxide in Huh7.5 cells. (A) Mitochondrial superoxide production in HCV-infected cells and the mock-infected control was examined at 6 days postinfection. Cells were directly incubated with MitoSOX (upper row) and then stained for HCV antigens by using an HCV-infected patient's serum, followed by FITC-labeled goat anti-human IgG (bottom row). Scale bar, 10 μm. (B) Quantification of MitoSOX-stained cells. The percentages of cells stained with MitoSOX were determined for HCV-infected cultures and the mock-infected control. Data represent means ± standard deviations of three independent experiments. *, P < 0.05, compared with the control.