Skip to main content
. 2008 Aug 25;28(21):6658–6667. doi: 10.1128/MCB.00738-08

FIG. 4.

FIG. 4.

Identification of the eIF3-binding domain of Paip1. (A) Schematic representation of Paip1 fragments used in GST pull-downs and Flag immunoprecipitation. Relative binding was evaluated visually. (B) GST pull-downs were conducted with the indicated GST-Paip1 fragments in HeLa cell extracts. Eluates were subjected to SDS-PAGE and Western blotting (WB) with the indicated antibodies. The GST and GST-tagged p65 proteins are indicated. (C) HeLa cells were transfected with plasmids expressing the indicated Flag-tagged Paip1 fragments or left untransfected (NT). Immunoprecipitations were performed 48 h posttransfection. Eluates were subjected to SDS-PAGE and Western blotting with the indicated antibodies.